Peptide reconstitution is the process of dissolving lyophilized (freeze-dried) peptide powder into bacteriostatic water to create an injectable solution. This guide walks through each step safely.
What You'll Need
Lyophilized peptide vial
Bacteriostatic water (BAC water)
Insulin syringes (1 mL, U-100)
Alcohol swabs
Sharps container
Steps
1. Gather supplies — Ensure sterile environment and all materials are ready
2. Swab the vial tops — Clean the rubber septum of both vials with an alcohol swab
3. Draw bacteriostatic water — Use a syringe to draw the target volume of BAC water
4. Inject water slowly — Insert needle into the peptide vial and inject water down the glass wall, not directly onto the powder
5. Swirl gently — Gently rotate the vial until the powder fully dissolves. Do not shake vigorously
6. Inspect the solution — The solution should be clear. Discard if cloudy or particulate
7. Label and refrigerate — Label with peptide name, concentration, and date. Store at 2–8°C